Multiplex immunoassay analysis of cytokines in idiopathic inflammatory myopathy

Arch Pathol Lab Med. 2008 Feb;132(2):232-8. doi: 10.5858/2008-132-232-MIAOCI.

Abstract

Context: Idiopathic inflammatory myopathies (IIMs), including dermatomyositis, polymyositis, and inclusion-body myositis, can be difficult to diagnose.

Objective: To determine if a multiplex immunoassay for markers of inflammation in muscle homogenates correlates with a diagnosis of IIM.

Design: Frozen archived muscle biopsy specimens from 30 patients with IIM and 34 patients without IIM were homogenized and analyzed for cytokine content with a multiplex microbead-based immunoassay system. Analyte concentrations were normalized to total lysate protein concentration prior to comparison.

Results: Two cytokines, interleukin 1ra and monocyte chemoattractant protein 1, and 1 soluble adhesion molecule, intracellular adhesion molecule 1, were found at significantly greater concentrations in muscle samples from patients with IIM. Intracellular adhesion molecule 1 levels alone were 83% sensitive and 91% specific for IIM at a cutoff of 1240 pg/mg muscle protein.

Conclusions: Immunoassays for selected inflammatory markers can serve in conjunction with histopathologic analysis as sensitive and specific tools for the diagnosis of IIM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Chemokine CCL2 / metabolism*
  • Child
  • Child, Preschool
  • Dermatomyositis / diagnosis
  • Dermatomyositis / metabolism*
  • Female
  • Humans
  • Immunoassay / methods
  • Infant
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Interleukin 1 Receptor Antagonist Protein / metabolism*
  • Male
  • Middle Aged
  • Muscle, Skeletal / metabolism
  • Myositis, Inclusion Body / diagnosis
  • Myositis, Inclusion Body / metabolism*
  • Polymyositis / diagnosis
  • Polymyositis / metabolism*
  • Sensitivity and Specificity

Substances

  • Chemokine CCL2
  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Intercellular Adhesion Molecule-1