Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart

Nature. 2008 Jan 31;451(7178):578-82. doi: 10.1038/nature06504.

Abstract

Understanding cellular response to environmental stress has broad implications for human disease. AMP-activated protein kinase (AMPK) orchestrates the regulation of energy-generating and -consuming pathways, and protects the heart against ischaemic injury and apoptosis. A role for circulating hormones such as adiponectin and leptin in the activation of AMPK has received recent attention. Whether local autocrine and paracrine factors within target organs such as the heart modulate AMPK is unknown. Here we show that macrophage migration inhibitory factor (MIF), an upstream regulator of inflammation, is released in the ischaemic heart, where it stimulates AMPK activation through CD74, promotes glucose uptake and protects the heart during ischaemia-reperfusion injury. Germline deletion of the Mif gene impairs ischaemic AMPK signalling in the mouse heart. Human fibroblasts with a low-activity MIF promoter polymorphism have diminished MIF release and AMPK activation during hypoxia. Thus, MIF modulates the activation of the cardioprotective AMPK pathway during ischaemia, functionally linking inflammation and metabolism in the heart. We anticipate that genetic variation in MIF expression may impact on the response of the human heart to ischaemia by the AMPK pathway, and that diagnostic MIF genotyping might predict risk in patients with coronary artery disease.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • AMP-Activated Protein Kinases
  • Animals
  • Antigens, Differentiation, B-Lymphocyte / genetics
  • Antigens, Differentiation, B-Lymphocyte / metabolism
  • Coronary Artery Disease / genetics
  • Enzyme Activation
  • Genetic Predisposition to Disease
  • Genotype
  • Glucose / metabolism
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / metabolism
  • Humans
  • Hypoxia / enzymology
  • Hypoxia / genetics
  • Hypoxia / metabolism
  • Macrophage Migration-Inhibitory Factors / deficiency
  • Macrophage Migration-Inhibitory Factors / genetics
  • Macrophage Migration-Inhibitory Factors / metabolism*
  • Mice
  • Multienzyme Complexes / metabolism*
  • Myocardial Ischemia / enzymology
  • Myocardial Ischemia / genetics
  • Myocardial Ischemia / metabolism*
  • Myocardial Reperfusion Injury / physiopathology
  • Myocardial Reperfusion Injury / prevention & control
  • Myocardium / enzymology
  • Myocardium / metabolism
  • Polymorphism, Genetic / genetics
  • Promoter Regions, Genetic / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Rats
  • Signal Transduction

Substances

  • Antigens, Differentiation, B-Lymphocyte
  • Histocompatibility Antigens Class II
  • Macrophage Migration-Inhibitory Factors
  • Multienzyme Complexes
  • invariant chain
  • Protein Serine-Threonine Kinases
  • AMP-Activated Protein Kinases
  • Glucose