Abstract
A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antibodies, Monoclonal / immunology
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Child
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Child, Preschool
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Fluorescent Antibody Technique, Direct / methods*
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Humans
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Infant
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Metapneumovirus / genetics
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Metapneumovirus / immunology
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Metapneumovirus / isolation & purification*
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Paramyxoviridae Infections / diagnosis*
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Paramyxoviridae Infections / virology
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Polymerase Chain Reaction / methods*
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Taq Polymerase
Substances
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Antibodies, Monoclonal
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Taq Polymerase