Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR

J Clin Microbiol. 2008 Mar;46(3):1098-100. doi: 10.1128/JCM.01926-07. Epub 2008 Jan 9.

Abstract

A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal / immunology
  • Child
  • Child, Preschool
  • Fluorescent Antibody Technique, Direct / methods*
  • Humans
  • Infant
  • Metapneumovirus / genetics
  • Metapneumovirus / immunology
  • Metapneumovirus / isolation & purification*
  • Paramyxoviridae Infections / diagnosis*
  • Paramyxoviridae Infections / virology
  • Polymerase Chain Reaction / methods*
  • Taq Polymerase

Substances

  • Antibodies, Monoclonal
  • Taq Polymerase