Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines

Ayesha B Alvero; Michele K Montagna; Gil Mor

doi:10.1007/978-1-59745-339-4_7

Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines

Methods Mol Biol. 2008:414:79-82. doi: 10.1007/978-1-59745-339-4_7.

Authors

Ayesha B Alvero¹, Michele K Montagna, Gil Mor

Affiliation

¹ Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.

PMID: 18175813
DOI: 10.1007/978-1-59745-339-4_7

Abstract

The immediate assessment of response to therapy is most beneficial to ovarian cancer patients. This study shows the correlation of drug-induced caspase activation determined by western blot analysis and by Caspase-Glotrade mark assay. Our findings demonstrate that the use of the Caspase-Glotrade mark assay allows a simple, fast, and sensitive alternative for the evaluation of in vitro response to chemotherapy.

MeSH terms

Antineoplastic Agents / therapeutic use*
Caspases / analysis
Caspases / metabolism*
Cell Line, Tumor
Female
Humans
Neoplasms, Glandular and Epithelial / drug therapy*
Neoplasms, Glandular and Epithelial / enzymology*
Neoplasms, Glandular and Epithelial / metabolism
Ovarian Neoplasms / drug therapy*
Ovarian Neoplasms / enzymology*
Ovarian Neoplasms / metabolism
Treatment Outcome

Substances

Antineoplastic Agents
Caspases