Riboswitch control of gene expression in plants by splicing and alternative 3' end processing of mRNAs

Plant Cell. 2007 Nov;19(11):3437-50. doi: 10.1105/tpc.107.053645. Epub 2007 Nov 9.

Abstract

The most widespread riboswitch class, found in organisms from all three domains of life, is responsive to the vitamin B(1) derivative thiamin pyrophosphate (TPP). We have established that a TPP-sensing riboswitch is present in the 3' untranslated region (UTR) of the thiamin biosynthetic gene THIC of all plant species examined. The THIC TPP riboswitch controls the formation of transcripts with alternative 3' UTR lengths, which affect mRNA accumulation and protein production. We demonstrate that riboswitch-mediated regulation of alternative 3' end processing is critical for TPP-dependent feedback control of THIC expression. Our data reveal a mechanism whereby metabolite-dependent alteration of RNA folding controls splicing and alternative 3' end processing of mRNAs. These findings highlight the importance of metabolite sensing by riboswitches in plants and further reveal the significance of alternative 3' end processing as a mechanism of gene control in eukaryotes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 3' Untranslated Regions / chemistry
  • 3' Untranslated Regions / genetics
  • Alternative Splicing / drug effects
  • Alternative Splicing / genetics*
  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / genetics
  • Base Sequence
  • Conserved Sequence
  • Exons / genetics
  • Feedback, Physiological / drug effects
  • Gene Expression Regulation, Plant* / drug effects
  • Introns / genetics
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation / genetics
  • Nucleic Acid Conformation / drug effects
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants / drug effects
  • Plants / genetics*
  • RNA 3' End Processing / drug effects
  • RNA 3' End Processing / genetics*
  • RNA, Messenger / genetics
  • Regulatory Sequences, Ribonucleic Acid / genetics*
  • Thiamine / pharmacology
  • Thiamine Pyrophosphate / metabolism

Substances

  • 3' Untranslated Regions
  • Aptamers, Nucleotide
  • Plant Proteins
  • RNA, Messenger
  • Regulatory Sequences, Ribonucleic Acid
  • Thiamine Pyrophosphate
  • Thiamine