A genetically engineered Escherichia coli, expressing the fusion protein of green fluorescent protein and carboxylesterase B1, can be easily detected in the environment following degradation of pesticide residues

Biotechnol Lett. 2007 Sep;29(9):1357-62. doi: 10.1007/s10529-007-9410-x. Epub 2007 Jun 21.

Abstract

Genetically engineered Escherichia coli, expressing the fusion protein of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), was successfully constructed by cloning the genes into the pET-28b vector and then transforming E. coli BL21 (DE3). Expression of the fusion protein was induced in E. coli BL21 (DE3) which could then degrade environmental pesticides and could be easily detected using fluorescence spectrophotometry or by the naked eye in daylight.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Biological Assay / methods
  • Biosensing Techniques / methods*
  • Carboxylic Ester Hydrolases / genetics
  • Carboxylic Ester Hydrolases / metabolism*
  • Environmental Monitoring / methods*
  • Escherichia coli / physiology*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • Pesticides / analysis
  • Pesticides / metabolism*
  • Protein Engineering / methods*
  • Recombinant Fusion Proteins / metabolism
  • Spectrometry, Fluorescence / methods

Substances

  • Pesticides
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Carboxylic Ester Hydrolases
  • carboxylesterase B1