Endothelial cell-T lymphocyte interactions: IP[corrected]-10 stimulates rapid transendothelial migration of human effector but not central memory CD4+ T cells. Requirements for shear stress and adhesion molecules

Transplantation. 2006 Jul 15;82(1 Suppl):S9-14. doi: 10.1097/01.tp.0000231356.57576.82.

Abstract

The chemokine interferon (IFN)-gamma-inducible protein of 10 kDa (IP-10; CXCL10) has been implicated in recruitment of T cells into rejecting allografts yet appears ineffective at stimulating human peripheral blood CD4 T cells to transmigrate across tumor necrosis factor (TNF)-treated human endothelial cell (EC) monolayers in vitro. The same cells rapidly (within 15 min) transmigrate across TNF-treated EC monolayers overlaid with stromal cell-derived factor-1 alpha (SDF-1 alpha) and subjected to shear stress. The effector memory subset within the CD4 T cell population, defined as CD45RO, CD62L and CCR7, which constitutes less than 10% of total CD4 T cells, does respond to IP-10 but requires enrichment to be observed in this model. Central memory T cells do not respond to IP-10. Transendothelial migration of effector memory CD4 T cells requires TNF-pretreatment of the EC monolayer and application of venular shear force during the assay. TNF treatment of ECs may be effectively replaced by transduction of vascular cell adhesion molecule-1 or intercellular adhesion molecule-1 but not E-selectin.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Adhesion
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / physiology*
  • Cell Communication / immunology*
  • Cell Movement / genetics
  • Cells, Cultured
  • Chemokine CXCL10
  • Chemokine CXCL12
  • Chemokines, CXC / pharmacology*
  • Endothelial Cells / drug effects
  • Endothelial Cells / immunology*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / immunology*
  • Graft Rejection / genetics
  • Graft Rejection / immunology*
  • Humans
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / physiology
  • L-Selectin / analysis
  • Leukocyte Common Antigens / analysis
  • Receptors, CCR7
  • Receptors, Chemokine / analysis
  • Stress, Mechanical
  • T-Lymphocyte Subsets / drug effects*
  • T-Lymphocyte Subsets / immunology
  • Transduction, Genetic
  • Tumor Necrosis Factors / pharmacology
  • Vascular Cell Adhesion Molecule-1 / genetics
  • Vascular Cell Adhesion Molecule-1 / physiology

Substances

  • CCR7 protein, human
  • CXCL12 protein, human
  • Cell Adhesion Molecules
  • Chemokine CXCL10
  • Chemokine CXCL12
  • Chemokines, CXC
  • Receptors, CCR7
  • Receptors, Chemokine
  • Tumor Necrosis Factors
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1
  • L-Selectin
  • Leukocyte Common Antigens