Assignment of the 1H chemical shifts of glycogen

Carbohydr Res. 1991 Nov 11:220:1-9. doi: 10.1016/0008-6215(91)80001-4.

Abstract

Assignments of nearly all the 1H chemical shifts of glycogen are made by 2-D 1H-1H homonuclear and 13C-1H heteronuclear COSY. We demonstrated that it is possible to obtain well-resolved 2-D n.m.r. spectra for a large molecule like glycogen. The seven nonequivalent protons of the glucose residues in the alpha-(1----4)-linked chains, and of those at the nonreducing ends, were completely assigned. Distinct chemical shifts for H-1 and H-2 immediately adjacent to the alpha-(1----6) bonds at the branch points were also determined. Several modifications of previous 13C chemical shift assignments were made from the heteronuclear 2-D n.m.r. data.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbon Isotopes
  • Deuterium
  • Deuterium Oxide
  • Glucose / chemistry
  • Glycogen / chemistry*
  • Hot Temperature
  • Isomaltose / chemistry
  • Magnetic Resonance Spectroscopy / methods*
  • Maltose / chemistry
  • Protons
  • Rabbits
  • Water

Substances

  • Carbon Isotopes
  • Protons
  • Water
  • Isomaltose
  • Maltose
  • Glycogen
  • Deuterium
  • Glucose
  • Deuterium Oxide