Microfluidic system for planar patch clamp electrode arrays

Nano Lett. 2006 Apr;6(4):815-9. doi: 10.1021/nl060165r.

Abstract

We present a microfluidic system integrated with disposable cell interface partitions for simultaneous patch clamp recordings. Glass-supported poly(dimethylsiloxane) (PDMS) partitions, having a 2 microm air-blown aperture, were reversibly sealed to a microfluidic system including PDMS channels with isolation valves and microfabricated Ag/AgCl electrodes. Gigaseal recordings from RBL-1 cells were obtained with a 24% success rate. Simultaneous whole cell recordings from valve-isolated electrodes were obtained.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Cell Line
  • Equipment Design
  • Equipment Failure Analysis
  • Flow Injection Analysis / methods
  • Mast Cells / physiology
  • Membrane Potentials / physiology*
  • Microelectrodes*
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Nanotechnology / instrumentation*
  • Nanotechnology / methods
  • Patch-Clamp Techniques / instrumentation*
  • Patch-Clamp Techniques / methods
  • Rats