Abstract
We present a microfluidic system integrated with disposable cell interface partitions for simultaneous patch clamp recordings. Glass-supported poly(dimethylsiloxane) (PDMS) partitions, having a 2 microm air-blown aperture, were reversibly sealed to a microfluidic system including PDMS channels with isolation valves and microfabricated Ag/AgCl electrodes. Gigaseal recordings from RBL-1 cells were obtained with a 24% success rate. Simultaneous whole cell recordings from valve-isolated electrodes were obtained.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cell Culture Techniques / instrumentation*
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Cell Culture Techniques / methods
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Cell Line
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Equipment Design
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Equipment Failure Analysis
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Flow Injection Analysis / methods
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Mast Cells / physiology
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Membrane Potentials / physiology*
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Microelectrodes*
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Microfluidic Analytical Techniques / instrumentation*
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Microfluidic Analytical Techniques / methods
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Nanotechnology / instrumentation*
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Nanotechnology / methods
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Patch-Clamp Techniques / instrumentation*
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Patch-Clamp Techniques / methods
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Rats