Abstract
One of the early steps in the biosynthesis of coenzyme F(420) in Methanocaldococcus jannaschii requires generation of 2-phospho-L-lactate, which is formed by the phosphorylation of L-lactate. Preliminary studies had shown that L-lactate in M. jannaschii is not derived from pyruvate, and thus an alternate pathway(s) for its formation was examined. Here we report that L-lactate is formed by the NAD(+)-dependent oxidation of l-lactaldehyde by the MJ1411 gene product. The lactaldehyde, in turn, was found to be generated either by the NAD(P)H reduction of methylglyoxal or by the aldol cleavage of fuculose-1-phosphate by fuculose-1-phosphate aldolase, the MJ1418 gene product.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aldehyde Oxidoreductases / chemistry
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Aldehyde Oxidoreductases / genetics*
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Aldehyde Oxidoreductases / isolation & purification
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Aldehyde Oxidoreductases / metabolism*
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Aldehydes / metabolism
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Amino Acid Sequence
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Archaeal Proteins / chemistry
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Archaeal Proteins / genetics*
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Archaeal Proteins / isolation & purification
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Archaeal Proteins / metabolism*
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Cloning, Molecular
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Escherichia coli / genetics
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Fructose-Bisphosphate Aldolase / genetics
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Fructose-Bisphosphate Aldolase / isolation & purification
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Fructose-Bisphosphate Aldolase / metabolism
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Hexosephosphates / metabolism
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Lactic Acid / metabolism*
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Methanococcales / enzymology*
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Methanococcales / genetics
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Molecular Sequence Data
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Pyruvaldehyde / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Riboflavin / analogs & derivatives*
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Riboflavin / biosynthesis
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Sequence Homology, Amino Acid
Substances
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Aldehydes
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Archaeal Proteins
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Hexosephosphates
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Recombinant Proteins
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fuculose 1-phosphate
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Lactic Acid
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lactaldehyde
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coenzyme F420
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Pyruvaldehyde
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Aldehyde Oxidoreductases
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lactaldehyde dehydrogenase
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Fructose-Bisphosphate Aldolase
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Riboflavin