Abstract
Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.
Publication types
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Research Support, N.I.H., Extramural
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Review
MeSH terms
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Animals
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Cell Membrane / chemistry
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Cell Membrane / ultrastructure
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Cryoelectron Microscopy / methods*
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Crystallography / methods
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Humans
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Image Processing, Computer-Assisted / methods
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Imaging, Three-Dimensional / methods*
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Macromolecular Substances*
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Ryanodine Receptor Calcium Release Channel / analysis
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Ryanodine Receptor Calcium Release Channel / ultrastructure
Substances
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Macromolecular Substances
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Ryanodine Receptor Calcium Release Channel