Genomic analysis of heat-shock factor targets in Drosophila

Genome Biol. 2005;6(7):R63. doi: 10.1186/gb-2005-6-7-r63. Epub 2005 Jun 10.

Abstract

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Embryo, Nonmammalian / physiology
  • Genomics*
  • Heat-Shock Proteins / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction / methods
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Transcription, Genetic

Substances

  • DNA Primers
  • Drosophila Proteins
  • Heat-Shock Proteins
  • Saccharomyces cerevisiae Proteins