Abstract
We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA Primers
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Drosophila Proteins / genetics*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Embryo, Nonmammalian / physiology
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Genomics*
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Heat-Shock Proteins / genetics*
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Oligonucleotide Array Sequence Analysis
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Polymerase Chain Reaction / methods
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins / genetics
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Transcription, Genetic
Substances
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DNA Primers
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Drosophila Proteins
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Heat-Shock Proteins
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Saccharomyces cerevisiae Proteins