Spectral-domain phase microscopy

Opt Lett. 2005 May 15;30(10):1162-4. doi: 10.1364/ol.30.001162.

Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Movement / physiology*
  • Cells, Cultured
  • Chick Embryo
  • Equipment Design
  • Equipment Failure Analysis
  • Microscopy, Interference / instrumentation*
  • Microscopy, Interference / methods
  • Microscopy, Phase-Contrast / instrumentation*
  • Microscopy, Phase-Contrast / methods
  • Myocardial Contraction / physiology*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / physiology*
  • Tomography, Optical Coherence / instrumentation*
  • Tomography, Optical Coherence / methods