Involvement of MEK1 MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, COI1 and CTR1 in N-mediated resistance to tobacco mosaic virus

Plant J. 2004 Jun;38(5):800-9. doi: 10.1111/j.1365-313X.2004.02085.x.

Abstract

The tobacco N gene, a member of the Toll-interleukin 1 homology region/nucleotide binding site/leucine-rich repeat (TIR-NBS-LRR) class of resistance (R) genes, confers resistance to tobacco mosaic virus (TMV). We used a candidate gene approach to identify known defense genes that were also involved in N signaling. The requirement for these genes was determined by downregulating their expression using the well-established tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS). Silencing of genes encoding a mitogen-activated protein kinase (MAPK) NTF6/NRK1, or an MAPK kinase (MAPKK) MEK1/NQK1, attenuated N-mediated resistance to TMV. We also found that N resistance is compromised in plants in which expression of WRKY1-WRKY3 and MYB1 transcription factors were downregulated. In addition, suppression of jasmonic acid (JA) signaling component COI1 ortholog affected N function. However, downregulation of expression of CTR1 ortholog leads to more rapid hypersensitive response (HR). The involvement of these genes in N- and other R-gene-mediated defense provides further evidence for the convergence of downstream signaling pathways of different R genes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Gene Silencing
  • Immunity, Innate / physiology*
  • MAP Kinase Kinase 1*
  • MAP Kinase Signaling System / genetics
  • MAP Kinase Signaling System / physiology
  • Mitogen-Activated Protein Kinase Kinases / genetics
  • Mitogen-Activated Protein Kinase Kinases / metabolism*
  • Mitogen-Activated Protein Kinases / genetics
  • Mitogen-Activated Protein Kinases / metabolism*
  • Molecular Sequence Data
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Proto-Oncogene Proteins c-myb / genetics
  • Proto-Oncogene Proteins c-myb / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tobacco Mosaic Virus / pathogenicity*
  • Transcription Factors / metabolism*
  • Transcription, Genetic

Substances

  • Plant Proteins
  • Proto-Oncogene Proteins c-myb
  • Transcription Factors
  • Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 1
  • Mek1 protein, Nicotiana tabacum
  • Mitogen-Activated Protein Kinase Kinases

Associated data

  • GENBANK/AY547492
  • GENBANK/AY547493
  • GENBANK/AY547494
  • GENBANK/AY547495
  • GENBANK/AY547496
  • GENBANK/AY547497
  • GENBANK/AY547498