Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site

Ole Vielemeyer; Jill Y Crouch; Stephen C Edberg; John G Howe

doi:10.1128/JCM.42.2.847-849.2004

Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site

J Clin Microbiol. 2004 Feb;42(2):847-9. doi: 10.1128/JCM.42.2.847-849.2004.

Authors

Ole Vielemeyer¹, Jill Y Crouch, Stephen C Edberg, John G Howe

Affiliation

¹ Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA.

Abstract

Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.

MeSH terms

AIDS-Related Opportunistic Infections / diagnosis*
Bordetella pertussis / genetics*
Bordetella pertussis / isolation & purification*
DNA, Ribosomal / genetics
Genotype
HIV Infections
Humans
RNA, Ribosomal, 16S / genetics
Rec A Recombinases / genetics*
Restriction Mapping / methods
Sputum / microbiology
Whooping Cough / diagnosis*

Substances

DNA, Ribosomal
RNA, Ribosomal, 16S
Rec A Recombinases