Abstract
The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Blotting, Western
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Cell Line
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Gene Expression / genetics
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial / genetics
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Oligonucleotides / genetics
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Oligonucleotides / metabolism*
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Phenotype
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Proton-Translocating ATPases / deficiency
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Proton-Translocating ATPases / genetics*
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Proton-Translocating ATPases / metabolism
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RNA, Bacterial / genetics
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RNA, Bacterial / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Untranslated
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Ribonuclease P / metabolism
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Salmonella typhimurium / genetics*
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Salmonella typhimurium / metabolism*
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Salmonella typhimurium / pathogenicity
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Substrate Specificity
Substances
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Bacterial Proteins
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Oligonucleotides
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RNA, Bacterial
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RNA, Messenger
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invB protein, Salmonella
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invC protein, Salmonella typhimurium
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Ribonuclease P
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Proton-Translocating ATPases
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RNA, Small Untranslated