CD154-CD40-independent up-regulation of B7-2 on splenic antigen-presenting cells and efficient T cell priming by staphylococcal enterotoxin A

Int Immunol. 2003 Jul;15(7):817-26. doi: 10.1093/intimm/dxg080.

Abstract

It has been demonstrated that in vivo T cell priming requires CD154-CD40 interaction, which is suggested to be critical in the induction of co-stimulatory activities on antigen-presenting cells (APC). In the current study, we demonstrate that in vivo administration of a high dose of a superantigen, staphylococcal enterotoxin A (SEA), could up-regulate B7-2 on most splenic APC independently of the CD154-CD40 interaction, followed by efficient expansion of SEA-reactive V(beta)3(+) T cells in CD154- or CD40-deficient mice. However, the CD154-CD40 interaction may be involved in SEA-mediated T cell activation, since a contribution of the CD154-CD40 interaction was observed when a lower dose of SEA was injected. CD154-independent T cell priming by SEA appeared also independent of the TRANCE-RANK pathway, which was shown to be capable of mediating CD154-independent activation of naive T cells during the infection of some viruses. These results indicate that SEA, which provokes rapid and efficient T cell responses without adjuvant, could utilize multiple CD154/TRANCE-independent pathways, to prime T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology*
  • Antigens, CD / biosynthesis*
  • B7-2 Antigen
  • CD40 Antigens / genetics
  • CD40 Antigens / physiology
  • CD40 Ligand / genetics
  • CD40 Ligand / metabolism
  • CD40 Ligand / physiology*
  • Enterotoxins / pharmacology*
  • Immunoglobulin Fc Fragments / immunology
  • Interferon-gamma / metabolism
  • Lymphocyte Activation*
  • Membrane Glycoproteins / biosynthesis*
  • Mice
  • Mice, Knockout
  • Spleen / cytology
  • Spleen / immunology*
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Up-Regulation

Substances

  • Antigens, CD
  • B7-2 Antigen
  • CD40 Antigens
  • Cd86 protein, mouse
  • Enterotoxins
  • Immunoglobulin Fc Fragments
  • Membrane Glycoproteins
  • CD40 Ligand
  • enterotoxin A, Staphylococcal
  • Interferon-gamma