A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis

Tian Wang; Louis A Magnarelli; John F Anderson; L Hannah Gould; Sandra L Bushmich; Susan J Wong; Erol Fikrig

doi:10.1089/153036602321131904

A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis

Vector Borne Zoonotic Dis. 2002 Summer;2(2):105-9. doi: 10.1089/153036602321131904.

Authors

Tian Wang¹, Louis A Magnarelli, John F Anderson, L Hannah Gould, Sandra L Bushmich, Susan J Wong, Erol Fikrig

Affiliation

¹ Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.

PMID: 12653304
DOI: 10.1089/153036602321131904

Abstract

Recombinant West Nile virus envelope (E) protein was examined in enzyme-linked immunosorbent assay (ELISA) to detect antibodies elicited during West Nile virus infection. Horses (nine of 10) and humans (six of six) with confirmed West Nile virus infection had IgG and/or IgM antibodies to the E protein. Antibodies to the recombinant West Nile virus membrane and nonstructural 1 proteins were not detected in any of these sera. An E protein-based ELISA may aid in the serological diagnosis of West Nile virus infection.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Antibodies, Viral / blood
Enzyme-Linked Immunosorbent Assay / methods*
Horses / immunology
Horses / virology
Humans
Immunoglobulin G / blood
Immunoglobulin M / blood
Recombinant Proteins / immunology
Viral Envelope Proteins / immunology*
West Nile Fever / diagnosis*
West Nile Fever / virology*
West Nile virus / immunology*
West Nile virus / isolation & purification*

Substances

Antibodies, Viral
Immunoglobulin G
Immunoglobulin M
Recombinant Proteins
Viral Envelope Proteins