Abstract
A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
AraC Transcription Factor
-
Arabinose / metabolism
-
Bacterial Proteins*
-
DNA-Directed RNA Polymerases / genetics*
-
Gene Expression Regulation, Bacterial*
-
Glucose / metabolism
-
Promoter Regions, Genetic*
-
RNA, Bacterial
-
Repressor Proteins / genetics
-
Salmonella typhimurium / genetics*
-
Transcription Factors*
-
Transcription, Genetic
-
Viral Proteins
Substances
-
AraC Transcription Factor
-
Bacterial Proteins
-
RNA, Bacterial
-
Repressor Proteins
-
Transcription Factors
-
Viral Proteins
-
Arabinose
-
bacteriophage T7 RNA polymerase
-
DNA-Directed RNA Polymerases
-
Glucose