Examination of the Borrelia burgdorferi transcriptome in Ixodes scapularis during feeding

J Bacteriol. 2002 Jun;184(11):3122-5. doi: 10.1128/JB.184.11.3122-3125.2002.

Abstract

Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachnid Vectors / microbiology*
  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics*
  • Blotting, Southern
  • Borrelia burgdorferi / genetics*
  • Borrelia burgdorferi / metabolism
  • Feeding Behavior
  • Gene Amplification
  • Gene Expression
  • Genomic Library
  • Ixodes / microbiology*
  • Ixodes / physiology
  • Mice
  • Mice, Inbred C3H
  • RNA, Bacterial / analysis
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • RNA, Messenger