Abstract
Interactions of LIGHT and its receptors, herpesvirus entry mediator on T cells and lymphotoxin beta receptor on stromal cells, are implicated in the regulation of lymphoid organogenesis, costimulation of T cells, and activation of dendritic cells. In this work we report that LIGHT-deficient mice had normal lymphoid organs with T cells and APCs that normally responded to Ag stimulation and normally stimulated T cells. Although the number of Vbeta8(+) T cells in naive LIGHT(+/+) and LIGHT(-/-) mice was identical, Vbeta8(+)CD8(+) T cell proliferation in response to staphylococcal enterotoxin B was significantly lower in LIGHT(-/-) mice. Consistently, induction and cytokine secretion of CD8(+) CTL to MHC class I-restricted peptide was also reduced in LIGHT(-/-) mice. However, the proliferative response of Vbeta8(+)CD4(+) T cells to staphylococcal enterotoxin B was comparable in LIGHT(-/-) and LIGHT(+/+) mice. Our results suggest that LIGHT is required for activation of normal CD8(+) T cells but not CD4(+) T cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibody Formation / genetics
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CD4-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / pathology
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Epitopes, T-Lymphocyte / immunology
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Female
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Gene Targeting / methods
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Haptens
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Hemocyanins / administration & dosage
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Hemocyanins / immunology
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Lymphocyte Activation / genetics
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Lymphoid Tissue / immunology
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Lymphoid Tissue / pathology
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Lymphotoxin beta Receptor
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Lymphotoxin-alpha / genetics
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Lymphotoxin-beta
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Male
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Membrane Proteins / biosynthesis
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Membrane Proteins / deficiency*
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Membrane Proteins / genetics*
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Membrane Proteins / physiology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Picrates / immunology
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Receptors, Tumor Necrosis Factor / deficiency
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Receptors, Tumor Necrosis Factor / genetics
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Tumor Necrosis Factor Ligand Superfamily Member 14
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / deficiency*
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Tumor Necrosis Factor-alpha / genetics*
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Tumor Necrosis Factor-alpha / physiology
Substances
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Epitopes, T-Lymphocyte
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Haptens
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Ltb protein, mouse
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Ltbr protein, mouse
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Lymphotoxin beta Receptor
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Lymphotoxin-alpha
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Lymphotoxin-beta
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Membrane Proteins
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Picrates
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Receptors, Tumor Necrosis Factor
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Tnfsf14 protein, mouse
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Tumor Necrosis Factor Ligand Superfamily Member 14
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Tumor Necrosis Factor-alpha
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trinitrophenyl keyhole limpet hemocyanin
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Hemocyanins