Abstract
A minor class of pre-mRNA introns whose excision requires a spliceosome containing U11, U12, U4atac/U6atac, and U5 snRNPs has been identified in plants, insects, and vertebrates. We have characterized single loci that specify the U6atac and U12 snRNAs of Drosophila melanogaster. P element-mediated disruptions of the U6atac and U12 genes cause lethality during the third instar larval and embryonic stages, respectively, and are rescued by U6atac and U12 transgenes. The P element disruption of U6atac results in excision defects of U12-type introns from several transcripts including an alternative U12-dependent spliced isoform of prospero, a homeodomain protein required for CNS development. Thus, we demonstrate the requirement for the U12 spliceosome in the development of a metazoan organism.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alternative Splicing
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Animals
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Animals, Genetically Modified
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Base Sequence
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Drosophila Proteins / genetics
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Drosophila Proteins / physiology*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics
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Drosophila melanogaster / growth & development
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Drosophila melanogaster / metabolism*
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Genes, Lethal
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Introns / genetics*
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Larva
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Molecular Sequence Data
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Mutagenesis, Insertional
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Nerve Tissue Proteins / genetics
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Nuclear Proteins / genetics
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Nucleic Acid Conformation
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Protein Isoforms / genetics
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RNA Precursors / genetics
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RNA Precursors / metabolism*
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RNA Splicing*
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RNA, Small Nuclear / genetics
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RNA, Small Nuclear / metabolism*
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Ribonucleoprotein, U4-U6 Small Nuclear / genetics
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Ribonucleoprotein, U4-U6 Small Nuclear / physiology*
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Ribonucleoproteins, Small Nuclear / genetics
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Ribonucleoproteins, Small Nuclear / physiology*
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Sequence Alignment
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Sequence Homology, Nucleic Acid
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Spliceosomes / physiology*
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Transcription Factors*
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Transgenes
Substances
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Drosophila Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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Protein Isoforms
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RNA Precursors
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RNA, Small Nuclear
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Ribonucleoprotein, U4-U6 Small Nuclear
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Ribonucleoproteins, Small Nuclear
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Transcription Factors
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U11-U12 small nuclear ribonucleoprotein
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U12 small nuclear RNA
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U6 small nuclear RNA
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pros protein, Drosophila
Associated data
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GENBANK/AF459089
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GENBANK/AF459090