Inhibition of attaching and effacing lesion formation following enteropathogenic Escherichia coli and Shiga toxin-producing E. coli infection

Infect Immun. 2001 Nov;69(11):7152-8. doi: 10.1128/IAI.69.11.7152-7158.2001.

Abstract

Enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) induce cytoskeletal changes in infected epithelial cells. To further characterize host cytosolic responses to infection, a series of specific cell-signaling inhibitors were employed. Initial bacterial adhesion to HEp-2 epithelial cells was not reduced, whereas alpha-actinin accumulation in infected cells was blocked by a phosphoinositide-specific phospholipase C inhibitor (ET-18-OCH3), phosphoinositide 3-kinase inhibitors (wortmannin and LY294002), and a 5-lipoxygenase inhibitor, nordihydroguaretic acid. A cyclooxygenase-2 inhibitor (NS-398), however, did not block alpha-actinin reorganization in response to EPEC and STEC infections. Understanding signal transduction responses to enteric pathogens could provide the basis for the development of novel therapeutic strategies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinin / metabolism
  • Androstadienes / pharmacology
  • Bacterial Adhesion / drug effects
  • Cell Line
  • Chromones / pharmacology
  • Cyclooxygenase 2
  • Cyclooxygenase 2 Inhibitors
  • Cyclooxygenase Inhibitors / pharmacology
  • Escherichia coli / isolation & purification
  • Escherichia coli / metabolism
  • Escherichia coli / pathogenicity*
  • Humans
  • Isoenzymes / metabolism
  • Lipoxygenase Inhibitors
  • Masoprocol / pharmacology
  • Membrane Proteins
  • Morpholines / pharmacology
  • Nitrobenzenes / pharmacology
  • Phosphoinositide-3 Kinase Inhibitors
  • Phospholipid Ethers / pharmacology
  • Prostaglandin-Endoperoxide Synthases / metabolism
  • Shiga Toxin
  • Signal Transduction / drug effects*
  • Sulfonamides / pharmacology
  • Type C Phospholipases / antagonists & inhibitors
  • Wortmannin

Substances

  • Androstadienes
  • Chromones
  • Cyclooxygenase 2 Inhibitors
  • Cyclooxygenase Inhibitors
  • Isoenzymes
  • Lipoxygenase Inhibitors
  • Membrane Proteins
  • Morpholines
  • Nitrobenzenes
  • Phosphoinositide-3 Kinase Inhibitors
  • Phospholipid Ethers
  • Sulfonamides
  • Actinin
  • N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
  • edelfosine
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Shiga Toxin
  • Masoprocol
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases
  • Type C Phospholipases
  • Wortmannin