In vitro evidence that immunuaffinity-purified MOG contains immunogenic quantities of contaminating mouse IgG; techniques for producing Ig-free MOG

J Neuroimmunol. 2001 Aug 30;118(2):194-202. doi: 10.1016/s0165-5728(01)00321-6.

Abstract

Myelin oligodendrocyte glycoprotein (MOG) has attracted considerable attention due to its possible role in multiple sclerosis (MS). Previous studies have shown that MOG-reactive T cells are more commonly detected in MS patients than controls. In this report, we studied human MOG-reactive T cell clones generated from healthy individuals as well as MS patients. Our results indicate that immunoaffinity-purified MOG, which was routinely used in prior studies, is contaminated by anti-MOG antibody (mouse IgG), despite the inability to detect IgG by Western blotting. Here, we used continuous SDS-PAGE, which enabled us to isolate highly purified MOG devoid of contaminating mouse IgG.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity / immunology
  • Blotting, Western
  • Cell Line
  • Clone Cells / cytology
  • Clone Cells / immunology
  • Clone Cells / metabolism
  • Cytokines / biosynthesis
  • Electrophoresis, Polyacrylamide Gel / methods
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Humans
  • Immunoglobulin G / chemistry
  • Immunoglobulin G / immunology*
  • Immunophenotyping
  • Immunosorbent Techniques*
  • Lymphocyte Activation / drug effects
  • Mice
  • Multiple Sclerosis / immunology*
  • Myelin Proteins
  • Myelin Sheath / chemistry
  • Myelin-Associated Glycoprotein / immunology*
  • Myelin-Associated Glycoprotein / isolation & purification*
  • Myelin-Associated Glycoprotein / pharmacology
  • Myelin-Oligodendrocyte Glycoprotein
  • Sensitivity and Specificity
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism

Substances

  • Antibodies, Monoclonal
  • Cytokines
  • Immunoglobulin G
  • MOG protein, human
  • Mog protein, mouse
  • Myelin Proteins
  • Myelin-Associated Glycoprotein
  • Myelin-Oligodendrocyte Glycoprotein