Abstract
Myelin oligodendrocyte glycoprotein (MOG) has attracted considerable attention due to its possible role in multiple sclerosis (MS). Previous studies have shown that MOG-reactive T cells are more commonly detected in MS patients than controls. In this report, we studied human MOG-reactive T cell clones generated from healthy individuals as well as MS patients. Our results indicate that immunoaffinity-purified MOG, which was routinely used in prior studies, is contaminated by anti-MOG antibody (mouse IgG), despite the inability to detect IgG by Western blotting. Here, we used continuous SDS-PAGE, which enabled us to isolate highly purified MOG devoid of contaminating mouse IgG.
MeSH terms
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Animals
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / immunology
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Antibody Specificity / immunology
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Blotting, Western
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Cell Line
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Clone Cells / cytology
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Clone Cells / immunology
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Clone Cells / metabolism
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Cytokines / biosynthesis
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Electrophoresis, Polyacrylamide Gel / methods
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Enzyme-Linked Immunosorbent Assay
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Flow Cytometry
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Humans
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Immunoglobulin G / chemistry
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Immunoglobulin G / immunology*
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Immunophenotyping
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Immunosorbent Techniques*
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Lymphocyte Activation / drug effects
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Mice
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Multiple Sclerosis / immunology*
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Myelin Proteins
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Myelin Sheath / chemistry
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Myelin-Associated Glycoprotein / immunology*
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Myelin-Associated Glycoprotein / isolation & purification*
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Myelin-Associated Glycoprotein / pharmacology
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Myelin-Oligodendrocyte Glycoprotein
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Sensitivity and Specificity
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism
Substances
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Antibodies, Monoclonal
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Cytokines
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Immunoglobulin G
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MOG protein, human
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Mog protein, mouse
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Myelin Proteins
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Myelin-Associated Glycoprotein
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Myelin-Oligodendrocyte Glycoprotein