Impact of sample type on rapid detection of influenza virus A by cytospin-enhanced immunofluorescence and membrane enzyme-linked immunosorbent assay

J Clin Microbiol. 2000 Jan;38(1):429-30. doi: 10.1128/JCM.38.1.429-430.2000.

Abstract

Cytospin-enhanced direct fluorescent-antibody assay (DFA) detected 49 (92.5%) and rapid membrane enzyme-linked immunosorbent assay (ELISA) detected 40 (75.5%) of 53 influenza virus A-positive samples. All 15 positive nasopharyngeal aspirates from children were detected by both tests. In contrast, 34 of 38 (89.5%) positive swabs from adults were detected by DFA, but only 25 (66%) were detected by ELISA.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Age Factors
  • Centrifugation
  • Child
  • Enzyme-Linked Immunosorbent Assay*
  • Fluorescent Antibody Technique, Direct*
  • Humans
  • Influenza A virus / isolation & purification*
  • Influenza, Human / diagnosis*
  • Lung / virology
  • Nasopharynx / virology
  • Specimen Handling