The citropin peptides from the skin glands of the Australian Blue Mountains tree frog Litoria citropa. Part 2: sequence determination using electrospray mass spectrometry

P A Wabnitz; J H Bowie; J C Wallace; M J Tyler

doi:10.1002/(SICI)1097-0231(19990915)13:17<1724::AID-RCM706>3.0.CO;2-T

The citropin peptides from the skin glands of the Australian Blue Mountains tree frog Litoria citropa. Part 2: sequence determination using electrospray mass spectrometry

Rapid Commun Mass Spectrom. 1999;13(17):1724-32. doi: 10.1002/(SICI)1097-0231(19990915)13:17<1724::AID-RCM706>3.0.CO;2-T.

Authors

P A Wabnitz¹, J H Bowie, J C Wallace, M J Tyler

Affiliation

¹ Department of Chemistry, The University of Adelaide, South Australia 5005.

Abstract

A combination of electrospray mass spectrometry, Lys-C digest/mass spectrometry and automated Edman sequencing provides the amino acid sequences of nineteen citropin peptides isolated from the granular dorsal and submental glands of the Blue Mountains tree frog Litoria citropa. Citropin 1.1 [Gly Leu Phe Asp Val Ile Lys Lys Val Ala Ser Val Ile Gly Gly Leu (NH(2))] and citropin 1.2 [Gly Leu Phe Asp Ile Ile Lys Lys Val Ala Ser Val Val Gly Gly Leu (NH(2))] are the two major skin peptides: both show significant wide-spectrum antibacterial activity.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amphibian Proteins*
Animals
Antimicrobial Cationic Peptides*
Anura / metabolism*
Australia
Mass Spectrometry
Molecular Sequence Data
Peptides / chemistry*
Sequence Analysis
Skin / chemistry*

Substances

Amphibian Proteins
Antimicrobial Cationic Peptides
Peptides
citropin 1.1 protein, Litoria
citropin 1.2 protein, Litoria