Abstract
Recently a number of nonclass I genes were discovered in the human MHC class I region. One of these, FAT10, encodes a protein consisting of two domains with homology to ubiquitin. FAT10 mRNA is expressed constitutively in some lymphoblastoid lines and dendritic cells and in certain other cells after gamma-interferon induction. FAT10 protein expression is controlled at several levels including transcription, translation, and protein stability. Yeast two-hybrid screening of a human lymphocyte library and immunoprecipitation studies revealed that FAT10 noncovalently associated with MAD2, a protein implicated in a cell-cycle checkpoint for spindle assembly during anaphase. Thus, FAT10 may modulate cell growth during B cell or dendritic cell development and activation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / immunology
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Base Sequence
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COS Cells
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Calcium-Binding Proteins*
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Cell Cycle Proteins
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Cell Line
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Chromosomes, Artificial, Yeast
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Genes, MHC Class I
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HL-60 Cells
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Humans
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Jurkat Cells
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Mad2 Proteins
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Molecular Sequence Data
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Polymerase Chain Reaction
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Repressor Proteins
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Sequence Alignment
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Sequence Homology, Amino Acid
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T-Lymphocytes / immunology
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Transfection
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Tumor Cells, Cultured
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Ubiquitins / chemistry
Substances
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Calcium-Binding Proteins
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Carrier Proteins
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Cell Cycle Proteins
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MAD2L1 protein, human
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Mad2 Proteins
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Repressor Proteins
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UBD protein, human
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Ubiquitins