Identification of a progenitor of the CTX-M-9 group of extended-spectrum beta-lactamases from Kluyvera georgiana isolated in Guyana

Adam B Olson; M Silverman; David A Boyd; Allison McGeer; Barbara M Willey; V Pong-Porter; N Daneman; Michael R Mulvey

doi:10.1128/AAC.49.5.2112-2115.2005

Identification of a progenitor of the CTX-M-9 group of extended-spectrum beta-lactamases from Kluyvera georgiana isolated in Guyana

Antimicrob Agents Chemother. 2005 May;49(5):2112-5. doi: 10.1128/AAC.49.5.2112-2115.2005.

Authors

Adam B Olson¹, M Silverman, David A Boyd, Allison McGeer, Barbara M Willey, V Pong-Porter, N Daneman, Michael R Mulvey

Affiliation

¹ University of Manitoba, Winnipeg, Canada.

Abstract

Chromosomal beta-lactamase genes (bla(KLUY)) from six Kluyvera georgiana strains isolated in Guyana were cloned and expressed in Escherichia coli. KLUY-1 exhibited 100% amino acid identity with the extended-spectrum beta-lactamase CTX-M-14. We also show that a 2.7-kb Kluyvera chromosomal region exhibits 99% nucleotide identity to a portion of In60 that includes bla(CTX-M-9).

MeSH terms

Amino Acid Sequence
Anti-Bacterial Agents / pharmacology
Chromosomes, Bacterial / genetics
Cloning, Molecular
Deoxyribonuclease EcoRI / chemistry
Escherichia coli / genetics
Escherichia coli Proteins / genetics*
Guyana
Humans
Kluyvera / drug effects
Kluyvera / enzymology*
Kluyvera / genetics*
Microbial Sensitivity Tests
Molecular Sequence Data
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
Deoxyribonuclease EcoRI
CTX-M-9 protein, E coli
beta-Lactamases

Associated data

GENBANK/AY623932
GENBANK/AY623933
GENBANK/AY623934
GENBANK/AY623935
GENBANK/AY824948