Abstract
Salmonella FliI is the ATPase that drives flagellar protein export. It normally exists as a complex together with the regulatory protein FliH. A fliH null mutant was slightly motile, with overproduction of FliI resulting in substantial improvement of its motility. Mutations in the cytoplasmic domains of FlhA and FlhB, which are integral membrane components of the type III flagellar export apparatus, also resulted in substantially improved motility, even at normal FliI levels. Thus, FliH, though undoubtedly important, is not essential.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biological Transport
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Flagella / metabolism*
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Gene Expression Regulation, Bacterial*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Point Mutation
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Proteins / metabolism*
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Proton-Translocating ATPases*
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Salmonella / genetics
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Salmonella / metabolism
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Salmonella / physiology*
Substances
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Bacterial Proteins
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FlhA protein, Bacteria
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FlhB protein, Bacteria
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Membrane Proteins
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Proteins
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fliH protein, Bacteria
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fliI protein, bacteria
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Adenosine Triphosphatases
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Proton-Translocating ATPases