Abstract
ISAba825, an insertion sequence found inactivating Acinetobacter baumannii carO, was tagged with a kanamycin (Kn) resistance cassette. ISAba825::Kn effectively transposed in A. baumannii, showing preference for short, AT-enriched target sequences, generating 6- to 9-bp target duplications. Additionally, we detected the presence of ISAba825 upstream of a plasmid-borne bla(OXA-58) gene, generating a hybrid promoter largely enhancing its expression and leading to carbapenem resistance. Overall, a role for ISAba825 in carbapenem resistance modulation in A. baumannii is proposed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter Infections / microbiology
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Acinetobacter baumannii / drug effects*
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Acinetobacter baumannii / enzymology
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Acinetobacter baumannii / genetics
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Acinetobacter baumannii / metabolism
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Anti-Bacterial Agents / pharmacology*
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Base Sequence
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Carbapenems / pharmacology*
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DNA Transposable Elements / genetics*
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DNA, Bacterial / genetics
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Gene Expression Regulation, Bacterial*
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Genome, Bacterial
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids / genetics
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Carbapenems
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DNA Transposable Elements
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DNA, Bacterial
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beta-Lactamases
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beta-lactamase OXA-58, Acinetobacter baumannii
Associated data
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GENBANK/HM068377
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GENBANK/HM068378