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1981 1
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1998 14
1999 5
2000 16
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66,281 results

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Page 1
Bacterial droplet-based single-cell RNA-seq reveals antibiotic-associated heterogeneous cellular states.
Ma P, Amemiya HM, He LL, Gandhi SJ, Nicol R, Bhattacharyya RP, Smillie CS, Hung DT. Ma P, et al. Cell. 2023 Feb 16;186(4):877-891.e14. doi: 10.1016/j.cell.2023.01.002. Epub 2023 Jan 27. Cell. 2023. PMID: 36708705 Free PMC article.
We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome many challenges hindering the development of scRNA-seq in bacteria. ...BacDrop thus can capture cellular states that cannot be detected by bulk RNA- …
We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome many challenges hinderi …
RNA-Seq Experiment and Data Analysis.
Withanage MHH, Liang H, Zeng E. Withanage MHH, et al. Methods Mol Biol. 2022;2418:405-424. doi: 10.1007/978-1-0716-1920-9_22. Methods Mol Biol. 2022. PMID: 35119677
Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncRNA, pre-mRNA) can also be investigated using RNA-Seq. ...In this chapter, we describe a protocol to construct an RNA- …
Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncR …
RNA-seq in Skeletal Biology.
Ayturk U. Ayturk U. Curr Osteoporos Rep. 2019 Aug;17(4):178-185. doi: 10.1007/s11914-019-00517-x. Curr Osteoporos Rep. 2019. PMID: 31093870 Review.
RNA-seq has led to the identification of novel genes and transcription factors involved in skeletal development and disease, through its application in small and large animal models, as well as human tissue and cells. With the availability of advanced techniques suc
RNA-seq has led to the identification of novel genes and transcription factors involved in skeletal development and disease, t
Processing and Analysis of RNA-seq Data from Public Resources.
Zoabi Y, Shomron N. Zoabi Y, et al. Methods Mol Biol. 2021;2243:81-94. doi: 10.1007/978-1-0716-1103-6_4. Methods Mol Biol. 2021. PMID: 33606253 Review.
Advances in next generation sequencing (NGS) technologies resulted in a broad array of large-scale gene expression studies and an unprecedented volume of whole messenger RNA (mRNA) sequencing data, or the transcriptome (also known as RNA sequencing, or RNA- …
Advances in next generation sequencing (NGS) technologies resulted in a broad array of large-scale gene expression studies and an unpreceden …
An RNA-Seq Protocol for Differential Expression Analysis.
Owens NDL, De Domenico E, Gilchrist MJ. Owens NDL, et al. Cold Spring Harb Protoc. 2019 Jun 3;2019(6). doi: 10.1101/pdb.prot098368. Cold Spring Harb Protoc. 2019. PMID: 30952685
Here we consider RNA-Seq, used to measure global gene expression through RNA fragmentation, capture, sequencing, and subsequent computational analysis. ...Here we present a standard RNA-Seq protocol for performing two-state differential gene exp …
Here we consider RNA-Seq, used to measure global gene expression through RNA fragmentation, capture, sequencing, and su …
Cross-species RNA-seq for deciphering host-microbe interactions.
Westermann AJ, Vogel J. Westermann AJ, et al. Nat Rev Genet. 2021 Jun;22(6):361-378. doi: 10.1038/s41576-021-00326-y. Epub 2021 Feb 17. Nat Rev Genet. 2021. PMID: 33597744 Review.
The cellular states of the interacting cells are decisive for the outcome of these encounters such as whether bacterial virulence programmes and host defence or tolerance mechanisms are induced. This Review summarizes how next-generation RNA sequencing (RNA-seq
The cellular states of the interacting cells are decisive for the outcome of these encounters such as whether bacterial virulence programmes …
Dual RNA-Seq of Mtb-Infected Macrophages In Vivo Reveals Ontologically Distinct Host-Pathogen Interactions.
Pisu D, Huang L, Grenier JK, Russell DG. Pisu D, et al. Cell Rep. 2020 Jan 14;30(2):335-350.e4. doi: 10.1016/j.celrep.2019.12.033. Cell Rep. 2020. PMID: 31940480 Free PMC article.
In this work, we explore the in vivo molecular dynamics of Mtb infection by performing dual RNA-seq on Mycobacterium tuberculosis-infected, ontogenetically distinct macrophage lineages isolated directly from murine lungs. ...
In this work, we explore the in vivo molecular dynamics of Mtb infection by performing dual RNA-seq on Mycobacterium tuberculo …
Single-nuclei RNA-seq on human retinal tissue provides improved transcriptome profiling.
Liang Q, Dharmat R, Owen L, Shakoor A, Li Y, Kim S, Vitale A, Kim I, Morgan D, Liang S, Wu N, Chen K, DeAngelis MM, Chen R. Liang Q, et al. Nat Commun. 2019 Dec 17;10(1):5743. doi: 10.1038/s41467-019-12917-9. Nat Commun. 2019. PMID: 31848347 Free PMC article.
Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with distinct functions. ...Furthermore, our dataset shows an improved power for prioritizing genes associated with huma …
Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of …
Current RNA-seq methodology reporting limits reproducibility.
Simoneau J, Dumontier S, Gosselin R, Scott MS. Simoneau J, et al. Brief Bioinform. 2021 Jan 18;22(1):140-145. doi: 10.1093/bib/bbz124. Brief Bioinform. 2021. PMID: 31813948 Free PMC article. Review.
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a biological sample. ...This work illustrates the need for a standardized and explicit display of methodological information in RNA-seq experiments....
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a biological sample. ...This work il …
66,281 results
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