FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO

J Biol Chem. 1998 Oct 16;273(42):27593-601. doi: 10.1074/jbc.273.42.27593.

Abstract

TFIIF (RAP30/74) is a general initiation factor that also increases the rate of elongation by RNA polymerase II. A two-hybrid screen for RAP74-interacting proteins produced cDNAs encoding FCP1a, a novel, ubiquitously expressed human protein that interacts with the carboxyl-terminal evolutionarily conserved domain of RAP74. Related cDNAs encoding FCP1b lack a carboxyl-terminal RAP74-binding domain of FCP1a. FCP1 is an essential subunit of a RAP74-stimulated phosphatase that processively dephosphorylates the carboxyl-terminal domain of the largest RNA polymerase II subunit. FCP1 is also a stoichiometric component of a human RNA polymerase II holoenzyme complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Cloning, Molecular
  • Holoenzymes / metabolism
  • Humans
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / metabolism*
  • Protein Binding
  • RNA Polymerase II / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Transcription Factors / metabolism*
  • Transcription Factors, TFII*
  • Transcription, Genetic

Substances

  • Holoenzymes
  • Transcription Factors
  • Transcription Factors, TFII
  • RNA Polymerase II
  • Phosphoprotein Phosphatases
  • transcription factor TFIIF

Associated data

  • GENBANK/AF081287