Abstract
The protein encoded by the human gene HEC (highly expressed in cancer) contains 642 amino acids and a long series of leucine heptad repeats at its C-terminal region. HEC protein is expressed most abundantly in the S and M phases of rapidly dividing cells but not in terminal differentiated cells. It localizes to the nuclei of interphase cells, and a portion distributes to centromeres during M phase. Inactivation of HEC by microinjection of specific monoclonal antibodies into cells during interphase severely disturbs the subsequent mitoses. Disordered sister chromatid alignment and separation, as well as the formation of nonviable cells with multiple, fragmented micronuclei, are common features observed. These results suggest that the HEC protein may play an important role in chromosome segregation during M phase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Differentiation
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Cell Division
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Cell Line
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Cell Nucleus / chemistry
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Cytoskeletal Proteins
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Haplorhini
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Humans
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Kidney / cytology
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Leucine
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Mice
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Mitosis*
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Molecular Sequence Data
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Molecular Weight
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Neoplasm Proteins / analysis
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / genetics
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Neoplasm Proteins / physiology*
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Neoplasms / chemistry
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Nuclear Proteins / analysis
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Organ Specificity
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RNA, Messenger / analysis
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RNA, Neoplasm / analysis
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Sequence Analysis, DNA
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Tumor Cells, Cultured
Substances
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Cytoskeletal Proteins
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NDC80 protein, human
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Neoplasm Proteins
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Nuclear Proteins
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RNA, Messenger
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RNA, Neoplasm
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Leucine