Abstract
Acetyl-CoA carboxylase and HMGCoA reductase are inactivated by the same AMP-activated protein kinase and are activated by type-2A protein phosphatase. To determine whether the same species of protein phosphatase-2A were involved, we studied the interconversion of acetyl-CoA carboxylase and HMGCoA reductase in isolated rat hepatocytes. We show that (i) these enzymes are differently regulated in hepatocytes and (ii) the species of type-2A protein phosphatase involved in their activation are different and can be separated by anion-exchange chromatography.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AMP-Activated Protein Kinases
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Acetyl-CoA Carboxylase / metabolism*
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Animals
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Cell Hypoxia
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Chromatography
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Enzyme Activation
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Glutamine / pharmacology*
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Hydroxymethylglutaryl CoA Reductases / metabolism*
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Liver / drug effects
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Liver / enzymology*
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Male
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Multienzyme Complexes / metabolism
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Phosphoprotein Phosphatases / metabolism*
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Protein Kinases / metabolism
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Protein Phosphatase 2
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Protein Serine-Threonine Kinases*
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Rats
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Rats, Wistar
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Time Factors
Substances
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Multienzyme Complexes
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Glutamine
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Hydroxymethylglutaryl CoA Reductases
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Protein Kinases
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Protein Serine-Threonine Kinases
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AMP-Activated Protein Kinases
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Acetyl-CoA Carboxylase