Transferable hyperproduction of TEM-1 beta-lactamase in Shigella flexneri due to a point mutation in the pribnow box

L K Siu; P L Ho; K Y Yuen; S S Wong; P Y Chau

doi:10.1128/AAC.41.2.468

Transferable hyperproduction of TEM-1 beta-lactamase in Shigella flexneri due to a point mutation in the pribnow box

Antimicrob Agents Chemother. 1997 Feb;41(2):468-70. doi: 10.1128/AAC.41.2.468.

Authors

L K Siu¹, P L Ho, K Y Yuen, S S Wong, P Y Chau

Affiliation

¹ Department of Microbiology, Queen Mary Hospital Compound, University of Hong Kong, Hong Kong.

Abstract

TEM-1 hyperproduction in two ampicillin-sulbactam-resistant Shigella flexneri strains was studied. In both strains the blaTEM gene was encoded as a single copy on a large conjugatively transferable plasmid. A single G-->T transversion at position 1 of the -10 consensus sequence was identified to be the mechanism of TEM-1 hyperproduction.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Base Sequence
Colony Count, Microbial
Drug Resistance, Microbial
Escherichia coli / genetics
Genes, Bacterial / genetics*
Molecular Sequence Data
Plasmids
Point Mutation
Polymerase Chain Reaction
Shigella flexneri / drug effects
Shigella flexneri / enzymology*
Shigella flexneri / isolation & purification
Transformation, Bacterial
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
beta-Lactamases
beta-lactamase TEM-1

Associated data

GENBANK/U48775