Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2

FEBS Lett. 1997 Jan 27;402(1):57-61. doi: 10.1016/s0014-5793(96)01500-1.

Abstract

We report the cDNA cloning, primary structure and tissue distribution of two new proteins homologous to mu-adaptins, the medium chains of the clathrin coat adaptor complexes. Both predicted proteins share 60% amino acid sequence identity with each other and 27-31%, identity with mu1-adaptin (ap47) and mu2-adaptin (ap50). Lower similarity (23-25% identity) is found with two other mu-adaptin-related proteins, p47A/B, and there is similarity over the N-terminal 150 amino acids with the adaptin small chains and deltaCOP. The mRNAs of both molecules are expressed in all tissues analyzed, but with different profiles of relative abundance. mu-ARP1 is most abundant in brain, ovary and lung, whereas mu-ARP2 is prominently expressed in testis. These proteins suggest the existence of as yet uncharacterized types of clathrin- or non-clathrin-associated protein coats in cellular membrane traffic, of which they are probably prototype subunits, and provide molecular markers and probes for their characterization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Protein Complex 1
  • Adaptor Protein Complex mu Subunits
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Chickens
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary / genetics
  • Female
  • Gene Expression
  • Humans
  • Lung
  • Male
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Organ Specificity
  • Ovary / metabolism
  • Testis / metabolism

Substances

  • Adaptor Protein Complex 1
  • Adaptor Protein Complex mu Subunits
  • Ap1m1 protein, mouse
  • DNA, Complementary
  • Membrane Proteins
  • mu-adaptin-related protein 2

Associated data

  • GENBANK/Y08386
  • GENBANK/Y08387