Gastrointestinal absorption of insulinlike growth factor-I in neonatal pigs

J Pediatr Gastroenterol Nutr. 1996 Nov;23(4):430-7. doi: 10.1097/00005176-199611000-00013.

Abstract

To determine if milk-borne insulinlike growth factor-I (IGF-I) is absorbed in neonatal piglets, recombinant human IGF-I was iodinated and then administered via an oral-gastric tube to newborn and 3-day-old piglets together with fluorescent isothiocyanate dextran (FITC-D70s, a macromolecular marker for assessment of gut closure). Results showed that total radioactivity and trichloroacetic acid (TCA)-precipitable radioactivity rose significantly in the plasma of both newborn and 3-day-old piglets 1 hour after oral-gastric administration of 125I-IGF-I, although the magnitude of increase was greater in newborns than in 3-day-old piglets. In contrast, significant absorption of FITC-D70s was observed only in newborn piglets but not in 3-day-old piglets. Chromatographic analysis revealed that 125I-IGF-I represented up to 20% of total plasma radioactivity in the newborns and up to 10% in the 3-day-old piglets. The 125I-IGF-I found in the plasma was mostly bound to IGF-binding proteins. These results indicate that milk-borne IGF-I can be absorbed in neonatal piglets and the absorption is independent of gut closure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn / metabolism*
  • Chromatography, Gel
  • Dextrans / metabolism
  • Fluorescein-5-isothiocyanate / analogs & derivatives
  • Fluorescein-5-isothiocyanate / metabolism
  • Humans
  • Insulin-Like Growth Factor Binding Proteins / blood
  • Insulin-Like Growth Factor I / metabolism*
  • Insulin-Like Growth Factor I / urine
  • Intestinal Absorption*
  • Iodine Radioisotopes
  • Recombinant Proteins / metabolism
  • Swine / metabolism*
  • Tissue Distribution

Substances

  • Dextrans
  • Insulin-Like Growth Factor Binding Proteins
  • Iodine Radioisotopes
  • Recombinant Proteins
  • fluorescein isothiocyanate dextran
  • Insulin-Like Growth Factor I
  • Fluorescein-5-isothiocyanate