Scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on the myocardium from the injury of ischemia-reperfusion

Biochim Biophys Acta. 1996 Mar 1;1315(2):131-7. doi: 10.1016/0925-4439(95)00112-3.

Abstract

The scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on myocardium from the ischemia-reperfusion injury was studied with electron spin resonance (ESR) and chemiluminescence techniques. Chinonin can effectively inhibit the oxidative activity of ONOO-, (the IC50 = 7 x 10 (-5) mmol/L) and scavenge oxygen free radicals generated from the reaction of xanthine and xanthine oxidase (the IC50 = 2/5 x 10(-4) mmol/l). It is difficult to find another antioxidant which can scavenge so effectively both ONOO- and oxygen free radicals simultaneously. In the system of ischemia-reperfusion injury of myocardium, Chinonin can, in parallel, scavenge the NO and oxygen free radicals generated from the ischemia-reperfused myocardium, and decrease the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart and therefore protect the heart from ischemia-reperfusion injury. The protective effect of 0.1 mmol/l Chinonin is similar to that of 1500 U/ml SOD and catalase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / analogs & derivatives
  • Arginine / pharmacology
  • Catalase / pharmacology
  • Electron Spin Resonance Spectroscopy
  • Enzyme Inhibitors / pharmacology
  • Free Radical Scavengers / pharmacology*
  • Free Radicals / metabolism
  • Glycosides / pharmacology*
  • Heart / drug effects*
  • Heart / physiology
  • Heart / physiopathology
  • Hemoglobins / metabolism
  • In Vitro Techniques
  • Kinetics
  • Luminescent Measurements
  • Male
  • Myocardial Ischemia / metabolism
  • Myocardial Ischemia / prevention & control*
  • Myocardial Reperfusion Injury / metabolism
  • Myocardial Reperfusion Injury / prevention & control*
  • Myocardium / metabolism*
  • NG-Nitroarginine Methyl Ester
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Oxidation-Reduction
  • Rats
  • Rats, Sprague-Dawley
  • Xanthenes / pharmacology*
  • Xanthine Oxidase / metabolism
  • Xanthones

Substances

  • Enzyme Inhibitors
  • Free Radical Scavengers
  • Free Radicals
  • Glycosides
  • Hemoglobins
  • Xanthenes
  • Xanthones
  • mangiferin
  • Nitric Oxide
  • Arginine
  • Catalase
  • Nitric Oxide Synthase
  • Xanthine Oxidase
  • NG-Nitroarginine Methyl Ester