Abstract
The scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on myocardium from the ischemia-reperfusion injury was studied with electron spin resonance (ESR) and chemiluminescence techniques. Chinonin can effectively inhibit the oxidative activity of ONOO-, (the IC50 = 7 x 10 (-5) mmol/L) and scavenge oxygen free radicals generated from the reaction of xanthine and xanthine oxidase (the IC50 = 2/5 x 10(-4) mmol/l). It is difficult to find another antioxidant which can scavenge so effectively both ONOO- and oxygen free radicals simultaneously. In the system of ischemia-reperfusion injury of myocardium, Chinonin can, in parallel, scavenge the NO and oxygen free radicals generated from the ischemia-reperfused myocardium, and decrease the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart and therefore protect the heart from ischemia-reperfusion injury. The protective effect of 0.1 mmol/l Chinonin is similar to that of 1500 U/ml SOD and catalase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arginine / analogs & derivatives
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Arginine / pharmacology
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Catalase / pharmacology
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Electron Spin Resonance Spectroscopy
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Enzyme Inhibitors / pharmacology
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Free Radical Scavengers / pharmacology*
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Free Radicals / metabolism
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Glycosides / pharmacology*
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Heart / drug effects*
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Heart / physiology
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Heart / physiopathology
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Hemoglobins / metabolism
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In Vitro Techniques
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Kinetics
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Luminescent Measurements
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Male
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Myocardial Ischemia / metabolism
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Myocardial Ischemia / prevention & control*
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Myocardial Reperfusion Injury / metabolism
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Myocardial Reperfusion Injury / prevention & control*
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Myocardium / metabolism*
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NG-Nitroarginine Methyl Ester
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Nitric Oxide / metabolism*
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Nitric Oxide Synthase / antagonists & inhibitors
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Oxidation-Reduction
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Rats
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Rats, Sprague-Dawley
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Xanthenes / pharmacology*
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Xanthine Oxidase / metabolism
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Xanthones
Substances
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Enzyme Inhibitors
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Free Radical Scavengers
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Free Radicals
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Glycosides
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Hemoglobins
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Xanthenes
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Xanthones
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mangiferin
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Nitric Oxide
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Arginine
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Catalase
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Nitric Oxide Synthase
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Xanthine Oxidase
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NG-Nitroarginine Methyl Ester