Refined localization and yeast artificial chromosome (YAC) contig--mapping of genes and DNA segments in the 7q21-q32 region

S W Scherer; J M Rommens; S Soder; E Wong; N Plavsic; B J Tompkins; A Beattie; J Kim; L C Tsui

doi:10.1093/hmg/2.6.751

Refined localization and yeast artificial chromosome (YAC) contig--mapping of genes and DNA segments in the 7q21-q32 region

Hum Mol Genet. 1993 Jun;2(6):751-60. doi: 10.1093/hmg/2.6.751.

Authors

S W Scherer¹, J M Rommens, S Soder, E Wong, N Plavsic, B J Tompkins, A Beattie, J Kim, L C Tsui

Affiliation

¹ Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.

PMID: 8353494
DOI: 10.1093/hmg/2.6.751

Abstract

The chromosome localizations for 159 gene and DNA segments have been refined to one of five intervals in the 7q21-132 region through hybridization analysis with a panel of somatic cell hybrid lines. Seventy-two of these chromosome 7 markers are also mapped on common or overlapping yeast artificial chromosome (YAC) clones. In addition, the breakpoints of chromosome rearrangement contained in five of the somatic cell hybrid lines have been defined by flanking probes within YAC contigs. To provide a framework for further mapping of the 7q21-q32 region, we have established the physical order of a set of reference markers: cen-(COL1A2-D7S15-CYP3A4-PON)-D7S456-(brea kpoint contained in cell hybrid 1EF2/3/K017)-GUSB-D7S186-ASL-(PGY1-PGY3 -GNB2-EPO-ACHE)-D7S238-(proximal breakpoint in GM1059-Rag5)-D7S240-(CUTL1-PLANH1)-(breakp oints in 1CF2/5/K016 and 2068Rag22-2)-(PRKAR2B-D7S13)-LAMB1-(breakpoint in JSR-17S)-DLD-D7S16-MET-WNT2-CFTR-D7S8-tel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chromosome Walking*
Chromosomes, Fungal*
Chromosomes, Human, Pair 7*
DNA / genetics
DNA Probes
Gene Library*
Genetic Markers
Genome, Human*
Humans
Hybrid Cells
Molecular Sequence Data

Substances

DNA Probes
Genetic Markers
DNA