Antibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodies

Anal Biochem. 1994 Feb 15;217(1):128-38. doi: 10.1006/abio.1994.1093.

Abstract

Affinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 x 10(-7) to approximately 3 x 10(-12) M. Dissociation constants for fluorescein and saxitoxin were compared with values determined by independent means. This technique was found to be quick, simple, reproducible, and accurate.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / metabolism*
  • Antibodies, Monoclonal
  • Antibody Affinity*
  • Antigen-Antibody Reactions*
  • Binding, Competitive
  • Fluorescein
  • Fluoresceins*
  • Immunoassay / methods
  • Kinetics
  • Potentiometry / methods
  • Ricin / immunology*
  • Saxitoxin / immunology*

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Fluoresceins
  • Saxitoxin
  • Ricin
  • Fluorescein