Abstract
alpha-Momorcharin, beta-momorcharin and trichosanthin increased the secretion of glutamate-pyruvate transaminase, lactate dehydrogenase and isocitrate dehydrogenase by isolated rat hepatocytes into the culture medium. This hepatotoxic effect of the proteins appeared to be concentration-dependent. The plant proteins induced morphological changes in hepatocytes including increased formation of cytoplasmic blebs and a reduction of microvilli on plasma membrane.
MeSH terms
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Alanine Transaminase / metabolism
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Animals
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Cells, Cultured
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Galactosamine / pharmacology
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Isocitrate Dehydrogenase / metabolism
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L-Lactate Dehydrogenase / metabolism
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Liver / drug effects*
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Liver / enzymology
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Liver / ultrastructure
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Male
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Microvilli / drug effects
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Plant Proteins / isolation & purification
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Plant Proteins / pharmacology*
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Plants, Medicinal / chemistry
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Rats
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Rats, Sprague-Dawley
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Ribosomal Proteins*
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Ribosome Inactivating Proteins
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Trichosanthin / isolation & purification
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Trichosanthin / pharmacology*
Substances
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Plant Proteins
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Ribosomal Proteins
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Trichosanthin
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Galactosamine
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MMC protein, Momordica charantia
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L-Lactate Dehydrogenase
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Isocitrate Dehydrogenase
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Alanine Transaminase
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Ribosome Inactivating Proteins