Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola

C Egli; W K Leung; K H Müller; R E Hancock; B C McBride

doi:10.1128/iai.61.5.1694-1699.1993

Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola

Infect Immun. 1993 May;61(5):1694-9. doi: 10.1128/iai.61.5.1694-1699.1993.

Authors

C Egli¹, W K Leung, K H Müller, R E Hancock, B C McBride

Affiliation

¹ Department of Microbiology, University of British Columbia, Vancouver, Canada.

Abstract

A 53-kDa protein from the outer sheath of the oral spirochete Treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. The channel had a single-channel conductance of 1.8 nS in 0.1 M KCl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). Electron micrographs of 53-kDa-protein-containing outer sheaths of T. denticola showed a regular hexagonal array of darker staining pits.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Bacterial / chemistry*
Antigens, Surface / chemistry*
Bacterial Adhesion
Bacterial Outer Membrane Proteins / chemistry*
Electric Conductivity
Ion Channel Gating
Ion Channels / chemistry*
Membranes, Artificial
Microscopy, Electron
Molecular Weight
Porins
Treponema / chemistry*
Treponema / ultrastructure

Substances

Antigens, Bacterial
Antigens, Surface
Bacterial Outer Membrane Proteins
Ion Channels
Membranes, Artificial
Porins