A serological study on the detection of HBV and HCV infection markers and the investigation of related sexual behaviors were conducted among 431 female prostitutes and 109 normal women. The result showed of anti-HBc prevalence rate was 43.1% (186/431), anti-HBs positive rate was 36.9% (159/431), HBsAg positive rate was 11.1% (48/431) and anti-HCV positive rate 9.51% (41/431). They were all significantly higher in prostitutes than in normal women. The infection indexes of HBV and HCV were also higher in population with STD than in normal women. The prevalence of anti-HCV in female prostitutes tended to increase with increase of age. There was a significant correlation with the number of their clients and years of being prostitutes. This demonstrated that there was a relatively high HBV and HCV infection rates in prostitutes.