CD5-positive and CD5-negative plaque-forming cells against poly-L-lysine-treated sheep erythrocytes in patients with systemic lupus erythematosus

Autoimmunity. 1994;18(3):189-94. doi: 10.3109/08916939409007995.

Abstract

While attempting to evaluate CD5+ and CD5- anti-DNA-secreting plaque-forming cells (PFC) in patients with systemic lupus erythematosus (SLE), significant numbers of PFC against control sheep erythrocytes (ShE) treated with poly-L-lysine (PLL) but not further conjugated with single-stranded (ss) or double-stranded (ds) DNA were noted. Numbers of PFC obtained using PLL-ShE, ssDNA-ShE and dsDNA-ShE were not significantly different, all reactivity to DNA apparently being accounted for by binding of antibodies to PLL-treated ShE. Nevertheless, anti-PLL-PFC could be inhibited by soluble dsDNA included in the plaque assay. These findings might be explained by cationic anti-DNA antibodies binding non-specifically to anionic PLL. Control healthy subjects gave few PFC against PLL-ShE, ssDNA-ShE or dsDNA-ShE. Anti-PLL-PFC appeared to be related to disease activity, with higher numbers of both CD5+ and CD5- PFC in patients with clinically active SLE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Antigens, CD / immunology*
  • Autoantibodies / biosynthesis*
  • B-Lymphocytes / immunology
  • CD5 Antigens
  • DNA / immunology
  • DNA, Single-Stranded / immunology
  • Erythrocytes / immunology
  • Female
  • Hemolytic Plaque Technique
  • Humans
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Pokeweed Mitogens / immunology
  • Polylysine / immunology*
  • Sheep
  • Staphylococcus aureus / immunology

Substances

  • Antigens, CD
  • Autoantibodies
  • CD5 Antigens
  • DNA, Single-Stranded
  • Pokeweed Mitogens
  • Polylysine
  • DNA