Abstract
The sequences of two previously defined tail genes, V and J, of the temperate bacteriophage P2, and those of two new essential tail genes, W and I, were determined. Their order is the late gene promoter, VWJI, followed by the tail fiber genes H and G, and a transcription terminator. The V gene product is the small spike at the tip of the tail, and the J gene product lies at the edge of the baseplate. The W gene product may be homologous to the product of gene 25 of T4 phage, which is part of the T4 baseplate. A temperature-sensitive mutation in gene V affects satellite phage P4 production more than it affects the production of P2 helper phage. P4 mutations that partially compensate for this defect of gene V lie in the P4 capsid size determination gene, sid.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Bacteriophage P2 / genetics*
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Bacteriophage P2 / physiology
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Bacteriophage P2 / ultrastructure
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Base Sequence
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Capsid / genetics
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Capsid / ultrastructure
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Cloning, Molecular
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DNA Primers / chemistry
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DNA, Viral / analysis
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation, Viral
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Genes, Viral / genetics*
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Genome, Viral*
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Molecular Sequence Data
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Mutation
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Rabbits
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Recombinant Proteins
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Transcription, Genetic
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Viral Proteins / genetics
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Viral Proteins / metabolism
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Viral Structural Proteins / genetics*
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Virus Assembly / physiology*
Substances
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DNA Primers
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DNA, Viral
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Recombinant Proteins
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Viral Proteins
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Viral Structural Proteins
Associated data
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GENBANK/L08879
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GENBANK/X51522
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GENBANK/X87173