Chemical modification of hemoglobin in intact whole cells: morphological studies by scanning electron microscope

Int J Biochem. 1982;14(6):461-5. doi: 10.1016/0020-711x(82)90113-6.

Abstract

1. Whole blood was incubated in physiological conditions with a variety of modifiers capable of modifying hemoglobin. Some of these compounds have been considered as therapeutic agents for certain hemoglobinopathies such as sickle-cell disease. 2. The extent of modification on hemoglobin was estimated by electrophoresis and the red-cell morphology by scanning electron microscope. 3. Results indicated that carbamyl phosphate, aspirin and glutaryl-salicylamide had no observable effect, whereas cross-linkers in general produced drastic changes in red-cell morphology. 4. Dibromoaspirin, a very effective acetylating agent, produced abnormalities even in concentrations as low as 1 mM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia, Sickle Cell / blood
  • Erythrocytes / drug effects
  • Erythrocytes / ultrastructure*
  • Hemoglobins, Abnormal / metabolism*
  • Hemolysis / drug effects
  • Humans
  • Microscopy, Electron, Scanning

Substances

  • Hemoglobins, Abnormal