Alterations in lysophosphatidylcholine levels of canine heart: modes of extraction and storage

Anal Biochem. 1984 Mar;137(2):277-81. doi: 10.1016/0003-2697(84)90085-x.

Abstract

The extraction of lysophosphatidylcholine from canine heart and the storage of lysophosphatidylcholine in total lipid extracts were investigated. The lysolipid was effectively extracted from canine heart by chloroform/methanol (1/2) and the maximum recovery of the lysolipid was 89-92%. Changes in levels of the lysolipid were observed when the tissue was stored at 0 degree C for 60 min or at -20 degrees C for 7 days. An alteration in the lysophosphatidylcholine level was also observed when the lipid extract from the heart was stored in theoretical lower phase (chloroform/methanol/water; 86/14/1). In order to accurately assess the level of lysophosphatidylcholine in the canine heart, the lipid should be extracted immediately from fresh tissue and the lipid extract stored in chloroform/methanol (2/1) or without solvent under nitrogen prior to separation and determination.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Dogs
  • Female
  • Lysophosphatidylcholines / isolation & purification*
  • Male
  • Myocardium / analysis*
  • Solvents
  • Specimen Handling
  • Time Factors

Substances

  • Lysophosphatidylcholines
  • Solvents