Cell-free synthesis of factor VIII related protein

Thromb Haemost. 1983 Dec 30;50(4):835-7.

Abstract

Polyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunoprecipitation, the translation product was found to contain 1.58% VIII related protein (VIII R), which has a molecular weight of 0.26 X 10(6), similar to the subunit of VIII R present in normal plasma. This indicated that VIII R messenger RNA is responsible for the synthesis of subunits only and intracellular post-translational events are required for the assembly of tetramer and multimers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / biosynthesis*
  • Antigens / genetics
  • Autoradiography
  • Cell-Free System
  • Electrophoresis, Agar Gel
  • Factor VIII / biosynthesis
  • Factor VIII / genetics
  • Factor VIII / immunology*
  • Humans
  • In Vitro Techniques
  • Poly A / isolation & purification*
  • Protein Biosynthesis
  • RNA / isolation & purification*
  • RNA, Messenger / isolation & purification*
  • von Willebrand Factor

Substances

  • Antigens
  • RNA, Messenger
  • von Willebrand Factor
  • Poly A
  • RNA
  • Factor VIII