Abstract
The binding of Tetrahymena 30S dynein to bovine brain microtubules has been examined. The dynein was bound by its B-end or ATP sensitive site with a stoichiometry of 1 dynein arm per 6 tubulin dimers. Addition of ATP released virtually all the dynein from the surface of the microtubule. Following hydrolysis of the ATP, the dynein reassociated with the microtubule.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Adenosine Triphosphate / pharmacology
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Alkaloids / pharmacology
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Animals
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Antineoplastic Agents, Phytogenic / pharmacology
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Binding Sites
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Brain / metabolism
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Cattle
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Dyneins / metabolism*
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Microtubules / metabolism*
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Paclitaxel
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Tetrahymena
Substances
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Alkaloids
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Antineoplastic Agents, Phytogenic
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Adenosine Triphosphate
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Adenosine Triphosphatases
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Dyneins
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Paclitaxel