Changes in adenylate cyclase and phosphodiesterase activities during the growth cycle of adult rat hepatocytes in primary culture

Arch Biochem Biophys. 1984 Aug 1;232(2):679-84. doi: 10.1016/0003-9861(84)90588-5.

Abstract

Long-term primary adult rat hepatocyte cultures show growth-state-dependent changes in adenylate cyclase and cAMP phosphodiesterase activities. Cellular adenylate cyclase activity decreases to undetectable levels within 1 day postplating, reappears on Days 4-5, and becomes maximal on Day 9. Membrane adenylate cyclase and cellular cAMP formation are insensitive to glucagon during log phase (Days 4-8) but not during lag (Day 1) or stationary phase (Day 12). Cyclic AMP phosphodiesterase activities (soluble and particulate) fall approximately equal to 70% by Day 2 but recover as proliferation begins. By contrast, the particulate phosphodiesterase assayed at 100 microM cAMP, decreased during Days 0-2. These observations simulate changes seen during liver proliferative transitions in vivo and, therefore, further support the use of these cultures as a developmental model.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3',5'-Cyclic-AMP Phosphodiesterases / metabolism*
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Cell Cycle
  • Cell Division
  • Cells, Cultured
  • Glucagon / pharmacology
  • Liver / cytology
  • Liver / enzymology*
  • Male
  • Rats
  • Rats, Inbred F344

Substances

  • Glucagon
  • 3',5'-Cyclic-AMP Phosphodiesterases
  • Adenylyl Cyclases